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Cell Culture – Multimedia

An Emerging Revolution in Automated 3D Cell Culture content piece image
Poster

An Emerging Revolution in Automated 3D Cell Culture

Market pressure exists for new paradigms to meet the demands for more rapid and reproducible cell production to keep up with advances in cell-based assays used in drug discovery and development. Novel automation platforms enable high productivity on three dimensional surfaces.
Long-term Gene Silencing in Mammalian Cells using siRNA content piece image
Poster

Long-term Gene Silencing in Mammalian Cells using siRNA

Gene silencing using siRNA-mediated RNAi has become a powerful tool in cell biology, addressing a range of research questions in functional genomics and drug discovery. After delivery of siRNA into the cell, rapid degradation of the target mRNA can be detected, often within 24 hours, and subsequently the corresponding protein is also knocked down.
High Throughput Cell Cycle Analysis using Microplate Cytometry content piece image
Poster

High Throughput Cell Cycle Analysis using Microplate Cytometry

To improve screening efficiency, we have developed a cell cycle analysis method that uses an Acumen Explorer fluorescence microplate cytometer to measure the DNA content of propidium iodide stained fixed cells in microplates. We demonstrate that paclitaxel and vinblastine arrested CHO cells in the expected phase of the cell cycle.
Detecting the FRET Response of the GeneBLAzer® Cell Line D1 CRE-bla CHO-K1 to Agonists and Antagonists using Microplate Cytometry content piece image
Poster

Detecting the FRET Response of the GeneBLAzer® Cell Line D1 CRE-bla CHO-K1 to Agonists and Antagonists using Microplate Cytometry

The GeneBLAzer® CHO.K1-D1 cell line (Invitrogen) stably expresses both the ß-lactamase gene downstream of the cAMP response element (CRE) and the dopamine D1 receptor. Stimulation of the cells with dopamine D1 receptor agonists, results in transcriptional activation of the ß-lactamase gene through CRE. A FRET-enabled substrate (CCF4-AM) fluoresces green, in the absence of ß- lactamase reporter activity, and blue when cleaved.
Design and Cellular Engineering content piece image
Poster

Design and Cellular Engineering

TRANSAT is a research partner for conception of cellular models. Its expertise lies in manipulation of gene expression (RNAi) and in mammalian cell culture. This expertise enables the company to propose functional genomics tools and customized research services, adapted to its clients' problematic. TRANSAT maintains its innovation through R&D programs.
LacZ Reporter Fusion Assay for Rapid and Easy Identification of Highly Efficient siRNA and its Delivery by Lentivirus into Suspension Cell Lines content piece image
Poster

LacZ Reporter Fusion Assay for Rapid and Easy Identification of Highly Efficient siRNA and its Delivery by Lentivirus into Suspension Cell Lines

The LacZ reporter fusion assay is an excellent method to precisely quantify knockdown efficiency of siRNA-sequences and can be corroborated by Western blot analysis. Efficient production of Lentiviruses and high transduction efficiency (up to 98%) were achieved on lymphoid B- and T-cells as demonstrated by FACS analysis and GFP expression. Successful knockdown effect with specific shRNA was observed in suspension cells three days after lentiviral infection.
Determination of Interferon-Pathway–Related Gene Induction during RNAi  content piece image
Poster

Determination of Interferon-Pathway–Related Gene Induction during RNAi

Experimental approaches using short interfering RNA (siRNA) molecules to specifically silence gene expression have become more widely used in recent years. As for all techniques in molecular and cellular biology, the importance of protocol optimization and the use of appropriate controls cannot be overestimated.
Development of High-Throughput PAMPA as an In Vitro Model of Passive Transcellular Permeation content piece image
Poster

Development of High-Throughput PAMPA as an In Vitro Model of Passive Transcellular Permeation

The absorption of orally administered compounds is largely determined by their ability to cross the gastrointestinal tract. Cell culture models can be intensive and limited to a narrow pH range. Assays using artificial membranes, such as PAMPA (parallel artificial membrane permeation assay) can be used as an alternative approach to assess in vitro transcellular passive permeation.
Monitoring Translocation Activity of Transcription Factors and Nuclear Hormone Receptors Using EFC content piece image
Poster

Monitoring Translocation Activity of Transcription Factors and Nuclear Hormone Receptors Using EFC

Enzyme Fragment Complementation can provide data for analysis of protein translocation events in whole cells, with the added benefits of increased throughput, simplified luminescence detection, no antibody requirements and decreased assay costs.
From Medicinal Plants to Bioactive Drugs content piece image
Poster

From Medicinal Plants to Bioactive Drugs

The aim of our study was to analyze the antiproliferative activity effects on human tumor cell lines and the differentiating activity on human erythroleukemic K562 cells of different extracts derived from medicinal plants of Bangladesh and Lebanon.
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