Western Blot – News and Features

The broad range of tumor types and their varying reactions to treatment make the search for new effective cancer therapies incredibly challenging. Non-invasive in vivo imaging technologies such as PET enable researchers to better understand the course of tumor progression, by visualizing cancer-related processes in real-time.

Antibodies are an essential part of everyday neuroscience research. But recently, high-profile criticism has cast doubt on their reliability and reproducibility. We caught up with Bio-Techne’s Raymond Chan and Katherine Bradley to find out how providers are hoping to improve antibodies and make them fit for a more reproducible future in neuroscience.

Having reliable antibodies can make or break your assay. However, sourcing the right antibody for the job can sometimes feel like you’re entering the lottery: there are so many on the market, and often they just don’t live up to what was promised. To learn more about antibody validation and its importance, we got in touch with Anthony Couvillon, PhD, Scientific Marketing Project Manager at Cell Signaling Technology.

The specificity of antibody binding is incredibly important for many research disciplines, yet sourcing the best antibody for your research can be a challenge. This is partly because not all suppliers validate their antibodies sufficiently. How much of a problem is this?

Having grown beyond early ‘proof-of-principle’ studies, top-down proteomics is now extensively used to analyze intact proteins in numerous applications. Here, we discuss how the top-down proteomics approach emerged, reasons underpinning its success, and highlight some of the most exciting current developments.

How does the gut talk to the brain? In this new case study from ProteinSimple, we find out how Melanie Maya Kaelberer, a Postdoctoral Associate at Duke University, is using Single-Cell Western platform Milo to answer the question of how the gut can rapidly communicate with cranial nerves.

This article outlines key workflow steps to help researchers perform better western blots. By executing best practices, researchers can expect good western blot data.

Presence of host cell protein (HCP) contaminants in the final product, even at very low levels, may elicit unpredictable and serious immunogenic response in humans. The accurate detection, identification and quantification of HCPs during biotherapeutic development is thus critical in ensuring patient safety, and is a regulatory requirement.

They say 'a picture is worth a thousand words.' Is this true for cell images?

A western blot is supposed to give you information on true experimental conditions or differences between samples. It is instrumental for the detection of proteins in research discovery and biopharma. In this article, we will be explaining the two main western blotting normalization methods; the older housekeeping protein method and the newer TPN method.