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Introducing Primer Extension Target Enrichment for NGS With Brian Godwin

Next-generation sequencing (NGS) target enrichment methods provide solutions to focus on and sequence a small portion of the human genome. This saves sequencing and analysis costs for labs that study genetic diseases. These savings typically range from approximately 100 to 100,000 fold, depending on the application and the amount of the genome being targeted. Until now, there have been two primary target enrichment methods used in the field.

Hybridization capture methods are considered high-performance solutions that tend to require cumbersome steps and an overnight hybridization incubation for optimal performance. On the other hand, amplicon technologies are considered fast and easy but generally do not have the same level of performance compared to capture technologies. Primer Extension Target Enrichment (PETE) is a newly developed technology that uses DNA polymerase extension reactions to provide a high-performance target enrichment solution that is easy to use and can be performed in a single day.

In this Teach Me in 10, Brian Godwin, director of reagent and assay development at Roche Sequencing, joins Lucy Lawrence to give us an overview of how each of the three target enrichment technologies work along. Godwin also shares a description of common target enrichment performance metrics.   

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