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Effective Nuclei Isolation From Tissue for Single-Nucleus Sequencing

Effective Nuclei Isolation From Tissue for Single-Nucleus Sequencing content piece image

Single-cell sequencing applications have accelerated breakthroughs in life sciences, leading to significant impacts for disease treatments.

Single-nuclei RNA-sequencing (snRNA-Seq) has been shown to be an acceptable substitute for scRNA-Seq in many applications. However, current protocols for isolating nuclei from complex tissues typically include labor-intensive, manual methods for mechanical disaggregation of the tissue and debris removal. These methods are not scalable to run multiple samples in parallel.

Learn how we can help you to generate high-quality and high-yield single-nucleus suspensions from a wide range of fresh and frozen tissues – otherwise seen as unsuitable samples – and achieve new heights in your genomic analysis.

In this webinar, we present a standardized workflow solution, including easy and automated nuclei extraction and isolation for reproducible single-nucleus sequencing analysis.

Attend this webinar to: 

  • Discuss and understand the importance of standardized sample preparation to ensure reliable single-nucleus analysis
  • Learn an easy method to isolate high quality nuclei from tissue in less than seven minutes from up to eight samples in parallel
  • Discover how to remove debris and clean up your nuclei samples and take a look at our single nucleus RNA-seq data
A picture of Ariadna Pascual Velazquez, PhD
Ariadna Pascual Velazquez, PhD
Product Manager for Molecular Analysis, Miltenyi Biotec B.V. & CO. KG