Molecular Diagnostics ddPCR for rapid RHD zygosity determination and highly sensitive non-invasive prenatal testing (NIPT) of fetal sex and RHD genotype
The use of DNA as a biomarker relies on analysis tools that are highly sensitive and give reproducible results. Digital PCR is a newer technology for DNA detection and quantification that is rapidly being adopted for applications in precision medicine. This seminar will cover the following topics:
- Digital PCR basics and assay development
- The application of digital PCR to disease diagnostics
- Digital PCR for highly sensitive non-invasive prenatal testing (NIPT)
- Cell-free DNA cancer testing with digital PCR
In this talk Dr Kelly Sillence will cover two pieces of her research undertaken whilst at Plymouth Univeristy. Firstly, she will cover RHD zygosity deterination in non-maternal samples for RHD zygosity to determine whether they have a single RHD, two copies of RHD or no copies of RHD a technique that is useful for blood transfusions and paternal testing. She will highlight the importance of determing paternal RHD status and discuss the assay design for ddPCR analysis of RHD zygosity.
Dr Sillence will then move on to discuss ddPCR and it's role in non-invasive prenatal testing (NIPT) of fetail sex and RHD genotype covering cell free fetal DNA (cffDNA) for non-invasive prenatal testing. She will also consider qPCR vs ddPCR for NIPT of fetal sex and RHD genotype.
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