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Precision qPCR and dPCR in the Discovery and Development of Cell and Gene Therapies

Precision qPCR and dPCR in the Discovery and Development of Cell and Gene Therapies content piece image
As a cornerstone of genetic analysis in biological research, quantitative PCR (qPCR) and digital PCR (dPCR) are the most sensitive molecular methods to analyze nucleic acids and play a key role in the development of cell and gene therapies (CGT).

These tools are widely used during CGT development for example in biodistribution, pharmacokinetics and pharmacodynamics of the investigational lead molecule. But many samples, including tissues rich in nucleases, lipids and other inhibiting substances and target molecules that are short or contain modified bases, can be challenging to work with. For these types of samples, assay design using advanced strategies, concentration standards, spike-in controls and validated normalization is key for reliable analyses.

In this webinar, Dr. Kubista will present strategies for implementing reliable qPCR and dPCR analysis in CGT development while highlighting best practices for adhering to MIQE and dMIQE guidelines and the recently released ISO standard, ISO 20395:2019.

Attend this webinar to:
  • Discover how qPCR and dPCR are used in the development of CGT
  • Understand best practice for qPCR and dPCR in CGT development
  • Explore recent developments in this field
A picture of Professor Mikael Kubista
Professor Mikael Kubista
Head of the Department of Gene Expression, Institute of Biotechnology, Czech Academy of Sciences