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16S rRNA Amplicon Sequencing Offers Enhanced Metagenomic Detection
Whitepaper

16S rRNA Amplicon Sequencing Offers Enhanced Metagenomic Detection

16S rRNA Amplicon Sequencing Offers Enhanced Metagenomic Detection
Whitepaper

16S rRNA Amplicon Sequencing Offers Enhanced Metagenomic Detection

Before the development of high-throughput methods to identify and characterize microbial populations, our understanding of the role microbes play in environmental, agricultural, and health-related settings was limited. ¬ e application of next generation sequencing (NGS) has provided an unprecedented ability to identify and categorize microbial taxonomy. Determining the complexity of species present in a sample can be achieved by sequencing a genomic region, conserved in all species, that contains evolutionarily divergent sequences that allow identifcation of unique taxa. A commonly used phylogenetic marker in metagenomics is the 16S ribosomal RNA (rRNA) gene. ¬ is ubiquitous locus is comprised of highly conserved regions  flanking nine hyper-variable regions, referred to as V1-V9 (Figure 1). Here we demonstrate the utility of the NEXTflex™ 16S V1-V3 Amplicon-Seq Kit combined with the longer read chemistry of Illumina MiSeq (2x300) for enabling accurate identification of genera present in highly complex microbial communities across a vast number of samples.  

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