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Latest Application Notes
Determining the Appropriate Sample Load When Using a Stain-Free V3 Western Workflow™
Application Note

Determining the Appropriate Sample Load When Using a Stain-Free V3 Western Workflow™

Traditional western blot workflows require an appropriate sample load to detect both target proteins and loading control proteins. The discrepancy between these quantities often leads to oversaturated protein bands, forcing researchers to sometimes run two duplicate gels or titrate down the antibody concentration in order to get quantitative results.
Determining the Appropriate Sample Load for Western Blots
Application Note

Determining the Appropriate Sample Load for Western Blots

Reliable western blot data can be generated only when the proper sample amount of protein is used. Loading too much protein leads to signal saturation in western blots, yet too little produces weak signals.
Avoiding Housekeeping Protein Detection Saturation
Application Note

Avoiding Housekeeping Protein Detection Saturation

Reliable western blot data require detection of the target and loading control proteins in the linear dynamic range. Many published western blot images show saturated protein bands, indicating that they were not measured in the linear dynamic range. Quantitative analysis based on this type of data is not reliable.
Automation of a Homogeneous Proximity Assay for Immunogenicity Testing of Biological Drug Products
Application Note

Automation of a Homogeneous Proximity Assay for Immunogenicity Testing of Biological Drug Products

Several challenges have surfaced during clinical evaluation of biological drug products due to a commonly associated immune response in patients. Anti-drug antibodies (ADA) are known to be frequently generated during administration of humanized monoclonal antibody therapeutics. A commonly used technology platform for assessment of immunogenicity relies on the bridging immunogenicity assay format typical of the ELISA.
Direct Visualisation, Sizing and Counting of Aggregation in Proteins
Application Note

Direct Visualisation, Sizing and Counting of Aggregation in Proteins

Characterising the state of aggregation in proteins is of paramount importance when trying to understand biopharmaceutical product stability and efficacy. Product quality, both in terms of biological activity and immunogenicity can be highly influenced by the state of protein aggregation.
Automated High Throughput Functional Cell-based Bioassays for EGFR Blocking Antibodies
Application Note

Automated High Throughput Functional Cell-based Bioassays for EGFR Blocking Antibodies

This study describes a novel, target-specific, cell-based assay platform that can be used to detect and differentiate potential inhibitors of Epidermal Growth Factor receptors.
Applications of rapid immunoassays (FAST ILA) in cell culture/fermentation and process development using the Threshold System
Application Note

Applications of rapid immunoassays (FAST ILA) in cell culture/fermentation and process development using the Threshold System

Accurate measurement of the concentration of a biological product is important in bioreactor/fermenter monitoring, downstream processing and final product quality control. Currently, electrophoretic, chromatographic and immunochemical methods are the most commonly used. These three methods are currently used in combination to provide the desired sample information.
Optimizing the Labeling of Proteins
Application Note

Optimizing the Labeling of Proteins

The Threshold® Immuno-Ligand Assay (ILA) adapts reagents used in ELISA, RIA or radioreceptor assays for the Threshold System by covalently attaching biotin or fluorescein labels to the binding proteins and/or ligands. To simplify labeling and purification of proteins, Molecular Devices has formulated biotin and fluorescein as N-hydroxysuccinimide esters.
The ELISAONE ™ Assay Performed on the POLARstar Omega from BMG LABTECH
Application Note

The ELISAONE ™ Assay Performed on the POLARstar Omega from BMG LABTECH

ELISAONE ™ technology has been developed by TGR BioSciences to provide a means of running high performance sandwich immunoassays in a user-friendly 96-well format.
Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules
Application Note

Promotion of Aggregation as a Means of Assessing the Stability of Antibody Molecules

During manufacture of antibody molecules, they are subjected to mechanical stress generated by processes such as pumping and filtration. This may cause denaturation and consequently aggregation due to exposure of the protein to air-liquid interfaces and shear forces, resulting in the ultimate loss in bioactivity.
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