Latest App Notes & Case Studies
App Note / Case Study
Characterization of IgG Monomers & Their Aggregates
In this application note we characterize two antibody samples using column calibration and multi-detection SEC.
App Note / Case Study
Understanding Antibody and Viral Glycoprotein Interactions Using CG-MALS
We used size-exclusion chromatography coupled with multi-angle light scattering to determine the molar mass and native oligo-meric state of a viral glycoprotein.
App Note / Case Study
Charge and Interaction Analysis for Predicting Antibody Formulation Stability
Colloidal stability is a key solution property of a biotherapeutic formulations, indicative of long-term stability. As it is readily measured over much shorter time scales than long-term stability testing or even accelerated testing regimens, measurements of colloidal stability are often utilized in the course of formulation work to screen out poorly behaved conditions.
App Note / Case Study
Performing ADCC Reporter Bioassay with an Environmentally Controlled Microplate Reader
The Promega ADCC Reporter Bioassay provides effector and target cells in frozen, thaw-and-use format, removing the need for isolating or culturing cells in order to perform the assay. Reporter induction requires a six-hour to overnight incubation in a standard cell culture incubator. The CO2 and temperature control features of the Tecan Infinite® 200 PRO with GCM allow the assay induction to be performed without the requirement for a cell culture incubator.
App Note / Case Study
Tag-lite® two-cell assay: a valuable tool for protein drug discovery
Antibodies are central mediators of the immune system, IgG being the most dominant. Binding of IgG/antigen complexes to membrane-bound Fc? receptors triggers a cellular immune response. Alterations in the Fc portion of the antibody by introducing mutations can lead to complete abolition of Fc? receptor binding.
This describes the development of a cellbased TR-FRET assay to determine the simultaneous binding of an antibody to its antigen and huFc?RIIIa present on different cells.
App Note / Case Study
Transcreener® ADP2 TR-FRET Red Assay
Adenosine diphosphate (ADP) is an important mediator of cellular metabolism that is essential for maintaining energy levels.
The Transcreener ADP2 TR-FRET Red Assay is intended for the detection of ADP production by any kinase or ATPase, and has been developed specifically for high throughput screening applications.
App Note / Case Study
Efficient washing of magnetic beads
The use of magnetic beads as the solid phase in a multiplexed ELISA enables high throughput cytokine screening and offers several advantages over common ELISA assays with direct coupling of the analyte to the well-surface.
App Note / Case Study
Automating ELISAs on Tecan’s Freedom EVO® using Optimiser™ technology from Siloam Biosciences
One of the challenges in life sciences research today is to discover methods for running key assays more quickly, more reliably and using lower volumes of reagents and sample, but still with improved sensitivity. One area where this particularly holds true is for traditional enzyme-linked immunosorbent assays (ELISAs), whose application provides a useful measurement of antigens, including cytokines and a host of other biomarkers.
App Note / Case Study
Validating the Expression Consistency of a Housekeeping Protein
Housekeeping proteins (HKP) are typically used as loading controls because it is assumed that the HKP expression level remains consistent across samples. However, there is evidence that HKP expression levels change in many scenarios, including siRNA treatment, cell death, cell differentiation, etc.
App Note / Case Study
A Method for Greater Reliability in Western Blot Loading Controls: Stain-Free Total Protein Quantitation
Reliable assessment of changes in target protein levels by western blot requires measurement of both the target and loading control proteins in the linear dynamic range. Stain-free technology is a novel method introduced by Bio-Rad to visualize and quantify proteins in gels and blots.
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