Transcreener® ADP2 TR-FRET Red AssayApplication Note
Tecan Group Ltd.
Adenosine diphosphate (ADP) is an important mediator of cellular metabolism that is essential for maintaining energy levels. The Transcreener ADP2 TR-FRET Red Assay is intended for the detection of ADP production by any kinase or ATPase, and has been developed specifically for high throughput screening applications.READ MORE
Efficient washing of magnetic beadsApplication Note
Tecan Group Ltd.
The use of magnetic beads as the solid phase in a multiplexed ELISA enables high throughput cytokine screening and offers several advantages over common ELISA assays with direct coupling of the analyte to the well-surface.READ MORE
Automating ELISAs on Tecan’s Freedom EVO® using Optimiser™ technology from Siloam BiosciencesApplication Note
Tecan Group Ltd.
One of the challenges in life sciences research today is to discover methods for running key assays more quickly, more reliably and using lower volumes of reagents and sample, but still with improved sensitivity. One area where this particularly holds true is for traditional enzyme-linked immunosorbent assays (ELISAs), whose application provides a useful measurement of antigens, including cytokines and a host of other biomarkers.READ MORE
Validating the Expression Consistency of a Housekeeping ProteinApplication Note
Housekeeping proteins (HKP) are typically used as loading controls because it is assumed that the HKP expression level remains consistent across samples. However, there is evidence that HKP expression levels change in many scenarios, including siRNA treatment, cell death, cell differentiation, etc.READ MORE
A Method for Greater Reliability in Western Blot Loading Controls: Stain-Free Total Protein QuantitationApplication Note
Reliable assessment of changes in target protein levels by western blot requires measurement of both the target and loading control proteins in the linear dynamic range. Stain-free technology is a novel method introduced by Bio-Rad to visualize and quantify proteins in gels and blots.READ MORE
General V3 Western Blotting ProtocolApplication Note
Western blotting is a very useful and widely adopted lab technique, but the traditional procedure can be long and tedious. Researchers can assess only whether a blot image is captured at the end of the long procedure and the quality of western blot data is sometimes challenged due to poor loading controls.READ MORE
Determining the Appropriate Sample Load When Using a Stain-Free V3 Western Workflow™Application Note
Traditional western blot workflows require an appropriate sample load to detect both target proteins and loading control proteins. The discrepancy between these quantities often leads to oversaturated protein bands, forcing researchers to sometimes run two duplicate gels or titrate down the antibody concentration in order to get quantitative results.READ MORE
Determining the Appropriate Sample Load for Western BlotsApplication Note
Reliable western blot data can be generated only when the proper sample amount of protein is used. Loading too much protein leads to signal saturation in western blots, yet too little produces weak signals.READ MORE
Avoiding Housekeeping Protein Detection SaturationApplication Note
Reliable western blot data require detection of the target and loading control proteins in the linear dynamic range. Many published western blot images show saturated protein bands, indicating that they were not measured in the linear dynamic range. Quantitative analysis based on this type of data is not reliable.READ MORE
Automation of a Homogeneous Proximity Assay for Immunogenicity Testing of Biological Drug ProductsApplication Note
BioTek Instruments Inc.
Several challenges have surfaced during clinical evaluation of biological drug products due to a commonly associated immune response in patients. Anti-drug antibodies (ADA) are known to be frequently generated during administration of humanized monoclonal antibody therapeutics. A commonly used technology platform for assessment of immunogenicity relies on the bridging immunogenicity assay format typical of the ELISA.READ MORE