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Determination of Sudan I and its Analogues in food

Sudan I is the common name given to 1-phenylazo-2-naphthol, a fat-soluble dye used industrially to colour oils, waxes, greases, varnishes etc. It has been recognised by health authorities as being potentially carcinogenic, and is banned as a food additive in the UK and across the European Union.  

This application note details how an HPLC (UV/Visible detection) method to analyse the four Sudan analogues in food samples has been developed.