Engineering Monoclonal Antibodies to Enhance Drugability: A Case Study

In this manuscript we compare the stability of three different engineered antibodies as determined by SEC-HPLC analysis during accelerated stability studies, with the stability predicted by DSC. The results demonstrate a correlation between decreased thermostabilty based on DSC data, and greater aggregation formation during accelerated stability studies for the antibodies studied. Thus, the results demonstrate how DSC can be a useful tool in stability screening of engineered proteins.