ENRICHMENT AND PURIFICATION OF CD3+ T-CELLS FROM HUMAN UMBILICAL CORD BLOOD
Application Note Jan 21, 2016
Quad Technologies’ has partnered with the University of Massachusetts Medical School (Worcester, MA, USA) to evaluate the performance of the MagCloudz™ Streptavidin cell separation kit. The goal of this study was to isolate and purify CD3+ T-cells from human umbilical cord blood (HUCB) for subsequent use in in vivo humanized mouse models. Here, we present representative data highlighting the enrichment, purity and viability of CD3+ T-cell populations isolated from HUCB using our MagCloudz™ Streptavidin cell separation kit with biotinylated-anti-CD3 antibody (clone UCHT1, eBioscience). Detailed protocols are available at www.quadtechnologies.com. Recovered T-cell populations were highly pure (>95 %), highly viable (> 94%) and magnetic-label free, which makes them ideal for further functional studies. Sustained viability and proliferation assays on recovered T-cells are currently underway.