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High Sensitivity Immunopeptidomics Using Mass Spectrometry

Peptide protein
Credit: iStock

Immunopeptidomics can reveal the diversity in major histocompatibility complex (MHC) bound proteins. However, sample diversity, low abundance and protein sequence variations, and complex data present challenges which require sophisticated mass spectrometry (MS) techniques. 

Trapped ion mobility spectrometry (TIMS) coupled with time of flight (TOF) methods can distinguish multiply charged ion signals with superior accuracy compared to conventional methods. Similar to the analysis of single-cell approaches, the timsTOF is an excellent tool for detecting low-concentration, diverse analytes in immunopeptidomics research. 

This application note demonstrates how timsTOF and single-cell proteomics (SCP) approaches can be combined to create a robust and sensitive immunopeptidomics workflow. 

Download this application note to explore: 

  • Preoptimized protocols for the detection of class I immunopeptides
  • timsTOF validation for accurate and robust results
  • The use of timsTOF SCP for peptide sequence comparison
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