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Optimized Protocol for ATCC Assay Ready THP-1 Monocyte Differentiation With PMA

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Credit: iStock

This protocol outlines the differentiation of ATCC Assay Ready THP-1 monocytes (ATCC® TIB-202-AR™) into macrophage-like cells using PMA, eliminating the need for prior cell culturing. It provides an optimized method for consistent macrophage differentiation, emphasizing aseptic techniques and immediate post-thaw seeding. The protocol can be used to obtain better differentiation into macrophage-like cells with high efficiency and consistency.