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Rapid Phosphoproteomics With DIA-PASEF

Rapid Phosphoproteomics With DIA-PASEF content piece image

Increasing the throughput and sensitivity of phosphoproteomics approaches is crucial to understanding the functional relevance of phosphorylation sites on a proteome-wide scale.

Data-independent acquisition (DIA) has been shown to increase the quantitative reproducibility and accuracy of MS-based phosphoproteomics. This app note explores the impact of dia-PASEF on the speed and sensitivity of phosphoproteomics.  

Download this app note to discover how you can:

  • Identify more than 12,500 phosphopeptides from low input material without a project-specific library
  • Detect over 9,300 phosphopeptides within a 7-minute gradient
  • Achieve increased reproducibility and data completeness