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The Development of a Sensitive and Reliable qRT-PCR-Based SARS-CoV-2 Wastewater Analysis Protocol

The Development of a Sensitive and Reliable qRT-PCR-Based SARS-CoV-2 Wastewater Analysis Protocol content piece image

Wastewater testing offers valuable insight on community-level data for various analytes, including pathogens that are associated with infectious diseases. Quantitative reverse transcriptase PCR (qRT-PCR) is well suited for wastewater analyses of pathogens due to its sensitivity, scalability and cost-effectiveness. It has been demonstrated that there is a correlation between SARS-CoV-2 RNA levels in wastewater and clinical cases, potentially providing predictive models for the community and healthcare institutions.

Download this app note to discover a method that can: 


  • Reliably determine SARS-CoV-2 viral RNA levels in a variety of raw wastewater samples
  • Provide high sensitivity
  • Detect as low as 8,000 viral genome copies per litre of wastewater