The ELISAONE ™ Assay Performed on the POLARstar Omega from BMG LABTECH
Application Note May 05, 2011
ELISAONE assays use a traditional immuno-sandwich format, but with a major difference. The analyte and both antibodies are added to the ELISAONE assay microplate at the same time, allowing solution-phase binding.
In contrast to other ELISA formats, the target-specific antibodies needed for the assay are not pre-bound to the microplate itself. The binding of antibodies to the analyte takes place in solution, allowing for efficient binding. This not only reduces assay times, but also affords several other benefits to the user. As the antibodies are not fixed to the plate, assays for several different targets can be performed in different wells on the same microplate, side-by-side. Another important benefit is reagent lifetime – many ELISA kits need to be discarded one month after opening, largely because the antibody-coated plates are often not stable after opening. With ELISAONE however, the expensive assay reagents are in reusable bottles, and last a long time after the ELISAONE microplate has expired. The inexpensive microplate can simply be discarded and another ordered, as they’re all identical.
This application note details how the ELISAONE assays for EGF, TNFα and IL-2 developed by TGR BioSciences, were used to demonstrate the robustness and high sensitivity of the ELISAONE technology.