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The New Gold Standard for Gene Therapy Protein Expression Potency Measurements

Protein Expression

Simple Western™: Analytical-Grade Protein Expression Quantification in a Multi-Attribute Platform Approach

A major challenge preventing transformative gene therapies from reaching patients is the lack of fit-for purpose potency assays, which are some of the most important assays in an analytical package but are often the most complex and high-risk.

Download this app note to discover a method with parallel line analysis for AAV-mediated gene delivery in cultured human cells. Specifically, this method demonstrates:

  • Relative protein expression potency between AAV serotypes with high specificity and <5% CVs
  • Absolute protein expression measurements in AAV transduced cells using a recombinant standard curve
  • Detection of residual intracellular capsid proteins in AAV transduced cells as an infectivity surrogate
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