DIA Vs DDA in Proteomics

Infographic

Published: September 3, 2019

Molly Campbell

DIA Vs DDA in Proteomics content piece image

Over recent years, liquid chromatography coupled to tandem mass-spectrometry (LC-MS/MS) has become the MS technology of choice for the high-throughput characterization of proteins and proteomes.

There are two modes of acquisition that can be used in LC-MS/MS experiments: the more traditional data dependent acquisition (DDA) mode, and the recently developed data independent acquisition (DIA) approaches, such as SWATH.

Download this infographic to gain an understanding of:

LC-MS/MS
The processes involved in an experiment using a DIA approach
How DIA produces a higher number of quantified proteins in a shorter analysis period than DDA

Meet the Author

Molly Campbell

Senior Science Writer

Molly Campbell is a senior science writer at Technology Networks. She holds a first-class honors degree in neuroscience. In 2021 Molly was shortlisted for the Women in Journalism Georgina Henry Award.