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A Guide to Sample Preparation for Next-Generation Sequencing

A sample tube held above a printed DNA sequence.
Credit: iStock

Next-generation sequencing (NGS) has become an indispensable tool for discovery, diagnosis, prognosis, forensics, precision medicine and more. NGS involves executing massively parallel sequencing of DNA or RNA in a fast, efficient, cost-effective and high-throughput manner.

The high sensitivity of NGS means that any compromise with the sample preparation quality can easily skew the results. 

Download this guide to explore tips on how to avoid issues during:

  • Experiment design
  • Tissue/specimen handling
  • Isolation of nucleic acids

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