The free publication discusses the differences between direct and indirect immuno-detection, various approaches to antibody labeling, and buffer formulations and antibody concentration. Also discussed is Innova Bioscience's Lightning Link, a simple and quick method for antibody labeling. The antibody labeling guide is also quick and concise; consisting of 9 informative pages.
Direct labeling of antibodies greatly simplifies immunodetection techniques. Without the problems of crossover and/or non-specific binding from secondary reagents it is far easier to obtain high quality results in techniques such as flow cytometry, ELISA, Western blotting and immunohistochemistry. A research group which has benefited from use of the Lightning-Link range is headed by Graham Pockley, the Professor of Immunobiology at the University of Sheffield, who commented:
‘My research depends heavily on multi-color flow cytometry and it was becoming increasingly difficult to commercially source the appropriate labeled antibodies for our studies. When I discovered the Lightning-Link technology I was really overwhelmed about the simplicity of the kits. More importantly, they were perfectly suited to our needs, as their ‘no loss’ labeling strategy means that we can purchase small aliquots of unlabeled antibody / isotype controls and label these in house. The ability to do this has transformed our work and I would highly recommend the kits to anyone in the research community.’