We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Dynamic Details of Virus Infection Cycle Revealed

Dynamic Details of Virus Infection Cycle Revealed

Dynamic Details of Virus Infection Cycle Revealed

Dynamic Details of Virus Infection Cycle Revealed

Virus Like Particles are mimics of the Nudaurelia capensis omega virus a model used to provide dynamic details about the process of viral maturation. Credit: Roger Castells-Graells.
Read time:

Want a FREE PDF version of This News Story?

Complete the form below and we will email you a PDF version of "Dynamic Details of Virus Infection Cycle Revealed"

First Name*
Last Name*
Email Address*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

A critical process in the infection cycle of viruses has been revealed for the first time in dynamic detail using pioneering plant-based technology.

Evidence about the process of maturation revealed in the research could help us develop new methods for treating viral infections.

To provide the first detailed mechanistic study of maturation, Roger Castells-Graells, a rotation Ph.D. student working in Professor George Lomonossoff’s laboratory at the John Innes Centre, infiltrated genetic material of the insect virus Nudaurelia capensis omega virus (N⍵V) into dwarf tobacco plants N.benthamiana.

This transient expression technique uses Virus Like Particles (VLPs) which are mimics of the authentic virus. The capsid or protein coat of the virus is expressed by plant cells and the research team then analyses the purified material from infiltrated leaves.

The research demonstrated that maturation of procapsids – immature viral structures – can occur within plant cells to yield fully functional mature capsids. This has not been observed previously in the absence of a natural infection and is a new application for the transient expression system pioneered by Professor George Lomonossoff at the John Innes Centre.

Comparative cryo-EM analysis of the structures of the procapsids and mature capsids revealed the large structural rearrangements both within and between the protein subunits of the capsid that accompany maturation and create the conditions necessary for viral replication and infection.

Professor George Lomonossoff said: “Most structural studies of virus particles to date have given a static picture of the particles. By isolating particles from plants that are undergoing the process of maturation, we have managed to obtain a picture of the dynamics of an essential part of a virus infection cycle.”

Maturation plays a critical role for all animal and bacterial viruses studied to date and is required to produce infectious virions or particles. Though the outlines of the process have been determined for many groups of viruses, detailed mechanistic studies have not been reported.

The present study, a collaboration involving scientists at the University of Leeds and colleagues in Brazil and the USA reveals details of the structures at the beginning and the end of the maturation process. What is now required is an analysis of intermediate steps to get a complete understanding of the dynamics.

This will enable the research team to determine the 3-D structures of intermediates in the maturation process to create a “movie.”

“We have shown that maturation occurs over time within plant cells and that means we have discovered a valuable tool for studying virus maturation. We hope it will be of interest to potential collaborators and industry,” said Professor Lomonossoff.

Castells-Graells R, Ribeiro JRS, Domitrovic T, et al. Plant-expressed virus-like particles reveal the intricate maturation process of a eukaryotic virus. Communications Biology. 2021;4(1):1-12. doi:10.1038/s42003-021-02134-w

This article has been republished from the following materials. Note: material may have been edited for length and content. For further information, please contact the cited source.