We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Lab-on-a-Chip Facilitates Bacterial Shape Sorting

Lab-on-a-Chip Facilitates Bacterial Shape Sorting

Lab-on-a-Chip Facilitates Bacterial Shape Sorting

Lab-on-a-Chip Facilitates Bacterial Shape Sorting

Digitally-colorized scanning electron micrograph (SEM) depicting numbers of Escherichia coli. Credit: National Institute of Allergy and Infectious Diseases
Read time:

Want a FREE PDF version of This News Story?

Complete the form below and we will email you a PDF version of "Lab-on-a-Chip Facilitates Bacterial Shape Sorting"

First Name*
Last Name*
Email Address*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

Scientists at Nara Institute of Science and Technology (NAIST) achieved shape-based separation of Escherichia coli (E. coli) bacteria using a viscoelastic fluid inside microfluidic channels. This "lab-on-a-chip" system has the capability to assist in making scientific experiments more reproducible, as well as provide more accurate assessments of the severity of bacterial infections based on patient samples.

The task of separating tiny bacteria based on their shape is a vital step in scientific and clinical applications, but also a difficult one. Whether an E. Coli bacterium is in a round or elongated configuration can indicate its state of biological function, and the ability to prepare homogeneous populations with uniform shapes may help improve experimental reproducibility. Moreover, being able to sort samples into sub-populations based on their shape may help diagnose patient health or assess environmental contamination. However, this ability has been difficult to achieve, especially at the scales needed to be practical.

Now, a team of researchers at NAIST and has been able to separate drug-treated E. coli bacteria using microfluidic channel containing poly-ethylene-oxide (PEO), a viscoelastic fluid. The elongation of the bacteria, as measured by the aspect ratio, ranged from 1.0 (spherical) to 5.5 (rod-shaped). Microchannels made of poly(dimethylsiloxane) were created with photolithography. PEO, which has both high viscosity and elasticity, was made to flow along the edges of an expanding V-shaped chamber. The elongated bacterium followed this flow to be collected at the edges of the device. "The shape of a E. coli bacterium changes based on its attachment, motility, dispersal, predation and differentiation behaviors," author Yalikun Yaxiaer explains. A rod-like configuration is more efficient for swimming, while a spherical shape may help it form communities. These changes are important milestones for the progression of disease, and whether the bacteria are swimming free or forming communities. "This technology will allow downstream genomics experiments on the separated E. coli populations to determine linkage between cell shape, antibiotic susceptibility and gene function," author Yoichiroh Hosokawa says. The team hopes the microfluidic platform they developed will be adopted by the broader scientific community for different applications in the fields of microbiology, molecular biology and biomedicine.

Reference: Zhang T, Liu H, Okano K, et al. Shape-based separation of drug-treated Escherichia coli using viscoelastic microfluidics. Lab Chip. 2022. doi: 10.1039/D2LC00339B

This article has been republished from the following materials. Note: material may have been edited for length and content. For further information, please contact the cited source.