Anti-CelTOS Transmission Blocking Activity in vivo and in vitro against P. falciparum by Epitope-specific Monoclonal Antibodies
Poster May 08, 2018
Shulin Xu, Alison E. Roth, Richard T. Luque, Nichole D. Salinas, Niraj H. Tolia, Samantha Barnes, Courtney Herman, John H. Adams.
Standard in vitro culture methods were used to produce mature gametocytes for the SMFA. The in vivo studies relied on an NF54 line carrying a luciferase-expressing cassette integrated in the genome. NSG mice treated with clodronate lipsome supported high-level engraftment of huRBC and can be infected by this P. falciparum luciferase reporter line, including development of mature gametocytes infectious for mosquitoes and leading to salivary gland sporozoites. This in vivo laboratory model permitted a highly sensitive transmission blocking assay to reliably quantify early oocyst development on day 22 post infection when stage V gametocytemia reached 0.07-0.18%. Mice were randomly divided into 4 groups and 60 min. before direct mosquito feed, each mouse in the antibody-treatment group received 16 mg/kg mAb in 200µl RPMI by iv injection and the blank control group received of equal volume RPMI. For the in vitro SMFA, 400 µg/ml of CelTOS mAb was added to gametocyte culture 60 min. prior to the mosquito feed. The presence of CelTOS mAb significantly inhibited oocyst development in mosquitoes in both in vivo and in vitro assays.
Young Adult Women’s Relationship Status and HPV Risk Perceptions: A Barrier to HPV Vaccination?Poster
Human papillomavirus (HPV) vaccine rates continue to be low in the United States. Young women ages 18-26 years are eligible for catch-up vaccination but previous research shows that relationships status and percieved risk may be barriers to HPV vaccination. The purpose of this quantitative study was to assess the association between relationship status and perceived risk for HPV among young adult women.READ MORE
Treatment Options for Chronic Parvovirus Viremia in Pediatric Heart Transplant Patients in a Tertiary Care CenterPoster
This abstract discusses three cases of pediatric heart transplant patients who suffered from parvovirus (B19) infection. Of these patients, two ( B & C) responded well to standard intravenous Ig therapy. Patient A however, did not respond to standard treatment and was begun on subcutaneous Ig, which effectively diminished his viral load. Thus, subcutaneous Ig infusions might serve as a second line treatment for transplant patients with parvovirus who do not respond well to the standard approach.READ MORE
T-helper cell phenotype expression in cutaneous lesions of angioimmunoblastic T-cell lymphomaPoster
Angioimmunoblastic T-cell lymphoma (AITL) is a common type of peripheral T-cell lymphoma. AITL can be missed until lymphadenopathy develops in patients initially presenting with skin lesions, as skin biopsy may lack conclusive findings. Our case highlights the extranodal presentation of AITL with cutaneous lesions displaying the TFH phenotype.READ MORE