Comparison of UVPD and ECD To Distinguish Between the Isomeric Products of Deamidation Using FT-ICR MS
Poster Nov 13, 2019
Anisha Haris, Yuko P. Y. Lam, Christopher A. Wootton, Alina Theisen, Cookson K. C. Chiu, Tomos E. Morgan, Mark P. Barrow, and Peter B. O’Connor
Deamidation of asparagine (Asn) is a spontaneous and non-enzymatic post-translational modification in proteins, playing a significant role in diseases related to aging such as Alzheimer’s and Parkinson’s disease. When this deamidation occurs, it results in isomeric aspartic acid (Asp) products.
To gain a better understanding of these products and the role they play, there are two methods that could be used to distinguish between isoAsp and Asp peptides: ultraviolet photodissociation (UVPD) and electron-capture dissociation (ECD).
To find out more about which of these methods is most suitable, download this poster, which details:
- A comparison of UVPD and ECD
- How FT-ICR MS can be used in this comparison
- The experimental methods used to compare