Nitrogen metabolism in Mycobacterium tuberculosis: a systems-based approach
Poster Apr 06, 2018
PiyaliS Basu, Dany JV Beste, KhushbooBorah, Kerstin J Williams, Johnjoe McFadden.
Several studies have already shown Mycobacterium tuberculosis (Mtb) obtains its carbon from host-derived nutrients and exploits specific metabolic pathways during infection.
In comparison to carbon, the processes Mtb uses to acquire and assimilate nitrogen (N) from the host is poorly studied despite N also being an essential component of biomolecules.
Our previous studies suggested the hypothesis that Mtb obtains N from a diverse range of intracellular nutrients including amino acids.
Here, we use a novel system’s based three-pronged approach to define pathways for uptake and assimilation of N. This consists of transposon mutagenesis, 15N isotopologueprofiling of intracellular Mtb, and performing flux analysis using 13C/15N isotopologue profiling in continuous culture.
Using this system’s strategy to study Mtb metabolism will help determine which N sources are required for ex vivo Mtb survival and the uptake and assimilation pathways involved to ultimately identify novel drug targets to treat TB.
Identification of genes involved in cell cycle regulation using arrayed synthetic CRISPR RNA libraries in a multiparameter high-content assayPoster
Gene knockout using CRISPR-Cas9 has dramatically transformed biological research and has been rapidly applied to loss-of-function screening primarily using pooled lentiviral sgRNA libraries. A synthetic CRISPR RNA (crRNA) approach is amenable to screening in arrayed, well-by-well fashion and expands the types of phenotypic readouts that can be used, including high-content and morphology-based assays.READ MORE
IntelliXtract 2.0: Simplified Intelligent Component Extraction and DetectionPoster
*Simplified detection and Component Extraction algorithm from LC-MS and GC-MS datasets
*New improved algorithm based on ion threads
*Reduced number of parameters to select for analysis
*Reduced false positives leading to reduced analysis time
Role of Elevated Airway Glucose (and Other Biochemicals) in Bacterial InfectionsPoster
Bacteria that live in the airways need something to eat: they mainly use host derived biochemicals, for example glucose. When levels of airway biochemicals are dysregulated, bacterial colonisation increases, enabling infection. We investigated how changes in airway glucose effect bacterial infection.READ MORE