Partial characterization of Plasmodium falciparum protein kinase ABCK2 (PfABCK2)
Due to their significance in the parasite lifecycle, one of the protein kinase of P. falciparum belongs to the ABC-1 family of proteins. The bioinformatic analysis and preliminary results of PfABCK2 showed the heterologous expression of this protein. Hence, the gene of PfABCk2 was ligated into pET21a+ vector with His-tag at C-terminus and transformed into BL-21 (DE3) competent cells that were verified through Miniprep and DNA sequencing. Furthermore, this gene construct is utilized to heterologous express this protein with IPTG and afterwards purified the recombinant protein kinase using nickel affinity chromatography as shown on 10% SDS-PAGE with the expected 36 kDa protein band. Therefore, the aim of this study is to partially characterize PfABCK2 protein kinase utilizing molecular cloning, heterologous express, protein kinase activity assay and targeted screening of protein kinase inhibitors.