Role of Elevated Airway Glucose (and Other Biochemicals) in Bacterial Infections
Poster Mar 07, 2018
Patrick Mallia, Simren Gill, Helen Groves, Hugo Farne, Sebastian Johnston, John S. Tregoning.
Bacteria that live in the airways need something to eat: they mainly use host derived biochemicals, for example glucose. Regulation of airway biochemicals is a host mechanism to control infection. When levels of airway biochemicals are dysregulated, bacterial colonisation increases, enabling infection. We investigated how changes in airway glucose effect bacterial infection.
Inflamed airways in patients with COPD may be enriched for biochemicals that support growth, leading to greater colonisation and infection, therefore reducing biochemical availability represents a new anti-bacterial strategy. In future we aim to investigate the effect of metformin on airway glucose in COPD.
Sport Doping Screening in Biological Matrices by Multi-Dimensional LC-QToFPoster
This work evaluated the performance of 2D LC variant using a QToF setup instead of a triple quadrupole mass spectrometer for the analysis of drug of abuse in urine targeting low and sub ppb level.READ MORE
Analysis of Doping and Forensic Drugs in Urine Using High-Resolution GC/Q-TOFPoster
In this study, we are examining the potential for high resolution accurate mass 7250 GC/Q-TOF equipped with low energy EI source, for both quantitative and screening aspects of doping control and forensic drugs applications.READ MORE
Characterization of a Type 2 diabetes-associated islet-specific enhancer cluster in STARD10 by genome editing of EndoC-βH1 cellsPoster
Genome-wide association studies (GWAS) have identified more than 100 genetic loci associated with type 2 diabetes. The majority of these are located in the intergenic or intragenic regions suggesting that the implicated variants may alter chromatin conformation. This, in turn, is likely to influence the expression of nearby or more remotely located genes to alter beta cell function. At present, however, detailed molecular and functional analyses are still lacking for most of these variants. We recently analysed one of these loci and mapped five causal variants in an islet-specific enhancer cluster within the STARD10 gene locus. Here, we aimed to understand how these causal variants influence b-cell function by alteration of the chromatin structure of enhancer clusterREAD MORE