Access 3D High-Plex Spatial Information Across Scales

Leica Microsystems, a leader in microscopy and scientific instrumentation, has released SpectraPlex, a 3D high-multiplex solution for spatial discoveries on the STELLARIS confocal platform. This groundbreaking innovation sets a new benchmark for 3D spatial phenotyping in life science research.

In recent years, Omics techniques – Genomics, Transcriptomics, Proteomics, and Metabolomics – have taken a central role in life science research. Understanding the spatiotemporal relationships between various biological components has become crucial for elucidating biological functions in both healthy and diseased states.

“SpectraPlex was developed to enable researchers to obtain more spatially resolved 3D data when interrogating complex disease states, allowing for a deeper analysis of pathological conditions”, said James O´Brien, Vice President of Life Sciences and Applied Solutions at Leica Microsystems. “The data acquired using SpectraPlex can propel the discovery of new cell types, the identification of cell states and the mapping of functional relationships in a 3D spatial context, aiding in the understanding of disease progression and the identification of potential therapeutic targets.”

With a fully integrated workflow, SpectraPlex enables scientists to capture detailed information at the right resolution and in 3D, with 15+ markers in one go, significantly surpassing conventional multicolor imaging. In addition, SpectraPlex offers an offline option to design experiments and explore optimal dye combinations to create panels for high-multiplex imaging. Defining a panel triggers real-time calculations, forming the basis for suggested microscope settings to maximize the signal-to-noise ratio and minimize crosstalk. Experienced users can still finetune these settings to accommodate sample-specific variances.

In SpectraPlex, the unmixed data along with the corresponding raw images is automatically generated to facilitate further analysis and interpretation. Users will benefit from tailored segmentation and downstream analysis for accurate interpretation of high-resolution, 3D data for over 15 labels, all powered by Aivia , Leica Microsystem’s cutting-edge AI image analysis software.

The next generation STELLARIS is a versatile, advanced confocal platform. Together with SpectraPlex and Aivia, it provides a fully integrated solution from imaging to analysis.