AnaSpec Introduces Anti-MOG (1-125)
Anti-MOG monoclonal antibody was produced by immunizing Balb/c mice with recombinant MOG (1-125) as antigen. It is supplied as purified mouse IgG1, 250 l in 40 mM MOPS (pH 7.5) with 50% glycerol containing 0.1% sodium azide and 0.1% BSA.
Myelin Oligodendrocyte Glycoprotein (MOG) is a member of the immunoglobulin superfamily and is expressed exclusively in the central nervous system (CNS). MOG protein constitutes only 0.01-0.5% of the CNS myelin proteins, has a mass of 27kDa, and is located in the outermost layer of myelin sheath (1-6). Mouse MOG is able to induce autoantibody production and relapsing-remitting neurological disease causing extensive plaque-like demyelination (1-4). Autoantibody response to mouse MOG has been observed in induced experimental autoimmune encephalomyelitis (EAE) in C57/BL6, 129/Sv, B6, and SJL mice (3-4).
AnaSpec has offered other MS research products, including MOG proteins, peptides, antibodies and assay kits.
The MOG proteins, with sequences corresponding to human, mouse and rat extracellular domain, together with a 6x His tag were expressed in E. coli and purified from urea denatured bacterial lysate. The molecular weight found in all 3 recombinant proteins was 14.23 kDa. These proteins were shown to induce EAE in either mouse (for the human and mouse rMOGs) or rat (for rat rMOG).
AnaSpec’s MOG (35-55) peptide from mouse and rat sequence was shown to induce anti-MOG (35-55) autoantibody production in C57BL/6 mice at 100% incidence (10/10); providing quantitative data that this peptide is suitable for in vivo anti-MOG (35-55) autoantibody study. A cysteine-containing MOG (35-55) peptide induced polyclonal anti-MOG (35-55) antibody production in rabbits with high specificity, high affinity, and high titer indicating its high purity, as well as suitability for use in peptide-coated ELISA and Western blot. This mouse/rat MOG (35-55) peptide was used as a coating peptide to develop the industry’s first anti-MOG (35-55) ELISA kit for EAE study. The detection limit of the kit was determined to reach as low as 50 pg of anti-MOG (35-55) autoantibody in serum or cerebrospinal fluid. Lot to lot variability of the MOG (35-55) was found to be minimal.
As for MOG Polyclonal Antibodies, one rabbit polyclonal antibodies towards the MOG extracellular domain raised against the human recombinant protein and the other against the mouse recombinant protein. These Protein A affinity purified antibodies recognized a 27kDa band.
The SensoLyte Anti-Human MOG (1-125) IgG Quantitation ELISA Kit, SensoLyte Anti-Mouse MOG (1-125) and SensoLyte Anti-Rat MOG (1-125) IgG ELISA assay kits are for determining the amount of anti-human MOG (1-125), anti-mouse MOG (1-125) and anti-rat MOG (1-125) antibodies. The improved SensoLyte® Anti -MOG(35 - 55) IgG Quantitative ELISA Kit (mouse/rat) is optimized to detect anti-MOG (35-55) mouse/rat IgG. Using strips pre-coated with MOG (35-55) peptide and pre-blocked with BSA, the amount of anti-MOG IgG subtypes in serum or cerebrospinal fluid can be determined by ELISA and the absorbance read at 450 nm.