AnaSpec Offers Industry’s First Anti-MOG ELISA Assay Kits
Product News Aug 23, 2011
AnaSpec to provide a whole suite of reagents and assay kits for the advancement of MS research. This includes MOG proteins, peptides, antibodies and assay kits.
Myelin Oligodendrocyte Glycoprotein (MOG), a protein with a molecular weight of 26- to 28-kDa, is a member of the immunoglobulin superfamily.
It is expressed exclusively in the central nervous system (CNS), specifically on the surface of myelinating oligodendrocytes and external lamellae of myelin sheath.
MOG protein constitutes only 0.01-0.05% of the CNS myelin proteins. However, it has been demonstrated to be a crucial autoantigen for multiple sclerosis (MS) in humans and experimental autoimmune encephalomyelitis (EAE) in rodents and monkeys.
These proteins, with sequences corresponding to human, mouse and rat extracellular domain, together with a 6x His tag were expressed in E. coli and purified from urea denatured bacterial lysate.
The molecular weight found in all 3 recombinant proteins was 14.23 kDa. These proteins were shown to induce EAE in either mouse (for the human and mouse rMOGs) or rat (for rat rMOG).