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Isolation of High Purity Exosomes

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AMSBIO is a leading supplier of high purity exosomes using isolation methods including traditional differential and gradient ultracentrifugation, immunoaffinity, size exclusion chromatography as well as precipitation / low speed centrifugation.

Exosomes are small endosome derived lipid nanoparticles actively secreted by exocytosis in most living cells. Release of exosomes occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Isolated from diverse cell lines, Exosomes have been shown to have pleiotropic physiological and pathological functions and play significant roles in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases.

Drawing upon many years’ experience - AMSBIO offers a range of tailored services for exosome isolation and characterization.

For labs requiring isolation and sequencing of exosome miRNA isolation, AMSBIO offers a service to extract RNA from exosomes, and subject it to RNA-Sequencing using an Illumina platform. In addition to basic analysis being performed on the raw data, screening for specific miRNA can also be performed. AMSBIO also offers analysis of exosome surface proteins using MACSPlex kit.

AMSBIO also offers a top-quality service that provides reliable and reproducible isolation of exosomes from almost any biofluid ranging from plasma and tissue culture media to cerebrospinal fluid. Using this service, your target exosomes are isolated by ultracentrifugation before quantification and quality assessment using a NanoDrop spectrophotometer.