The PhysioMimix™ OOC Range of Single and Multi-Organ Microphysiological Systems
After years of costly research and development, many drug discovery programs fail because 2D cell culture and animal models fail to adequately predict human responses. By recreating human physiology in the lab, the purpose of Organ-on-a-chip (OOC) is to help mitigate the risk of late-stage clinical failures by delivering fast, accurate and clinically translatable data in response to new therapeutic drug candidates. The insights generated using OOC complement those obtained using traditional methods to confirm discoveries, overcome inter-species differences, and bridge knowledge gaps.
Culturing multiple human cell types in a physiologically relevant environment, the PhysioMimix™ OOC range of Single-, and Multi-Organ Microphysiological Systems (MPS) generate 3D microtissues, or miniature organs in the lab, that behave and respond to drugs in the same way as a human. These systems maintain the phenotype and biological activity of cultures over weeks by mimicking blood flow around the human body, exposing cells to vital biomechanical stimuli, oxygen and nutrients.
The PhysioMimix™ Single-Organ System provides detailed individual organ function and disease state modelling studies, using the MPS-LC12 consumable plate to generate in vitro 3D liver microtissues that mimic the human liver microarchitecture, or the MPS-T12 consumable plate to develop barrier models (such as gut or lung) on Transwell® inserts.
In addition, the Multi-Organ System enables scientists to interconnect our liver-on-a-chip model with other organs, such as gut, lung or kidney. By mimicking how organs interact and communicate as part of a complex system, researchers benefit from a deeper human-specific mechanistic understanding of disease states and drug behaviour such as the ability to analyse target organ versus off-target effects, pharmacokinetics, bioavailability and biodistribution. This capability is facilitated by the MPS-TL6 consumable plate whose novel microfluidics enable recirculation of media from one culture compartment to the other to permit inter-organ crosstalk. When combined with PBPK mathematical modelling, in vitro data may be extrapolated to in vivo clinical predictions.
Key applications include:
- Disease modelling
- Safety toxicology
- ADME
Key PhysioMimix™ OOC features include:
- Compatible with existing lab equipment: no additional purchases necessary
- Easy to adopt: supports users transitioning from 2D culture with a familiar plate-based consumable
- Simple to program: < 1 min to start an automated run over many weeks
- Recirculated microfluidic flow: delivers biomechanical stimulus, oxygen and nutrients to cells retaining viability and phenotype
- Versatile: develop your own models or follow our validated SOPs
- Flexible: organoids, spheroids, iPSCs, primary cells, immortalized cell lines, tissue slices and commercial inserts
- Multi-organ capability: link two models using microfluidics to reveal multiple organ interactions and response to stimuli
- Adjustable inter- and intra- “organ-specific” flow rates: enhance physiological relevance
- Real-time monitoring: remove samples for analysis while experiments continue to run
- Large sampling scale: high content data generation for deep -omics and microscopic analysis
- Medium throughput: simultaneously run up to six independent experiments
- Lab benchtop ready: portable and compact
- PDMS-free consumable plates: ensure low non-specific binding for true assessment of drug response
