TruCulture® - Standardized Functional Immunophenotyping

TruCulture whole blood collection and stimulation system has revolutionized immune monitoring, especially for pharmacodynamic Phase 1 clinical trials. TruCulture tubes are pre-loaded with proprietary cell culture media and immune stimulant(s) or drug candidates. At the clinical site, blood is drawn directly from a subject into the tube for an instant initiation of the ex vivo stimulation. After incubation in a dry heat block, cells are simply separated from the culture supernatant with a valve separator.

TruCulture tubes offer the following benefits and downstream analysis:

• Numerous Stimulants— Investigate immune responses to bacterial, viral, T cell cytokine activators with or without drug candidate
• Reduce Variability—eliminates sample processing & delay prior to culturing
• Reproducible — easy to use, integrated, closed sterile system
• Use Anywhere—only requires a 37° heat block and phlebotomist
• Biomarker Analysis— determine functional immune cell activity with Myriad RBM’s OptiMAP panel, designed to measure key secreted proteins from TruCulture supernatants
• Vaccine Development— antigen recall analysis and profile immune status of patients
• Flow Cytometry— protocol available for surface marker expression of cell populations
• Gene Expression— RNA extraction protocols available