Cell Culture – Multimedia

Poster
High Throughput Cell Cycle Analysis using Microplate Cytometry
To improve screening efficiency, we have developed a cell cycle analysis method that uses an Acumen Explorer fluorescence microplate cytometer to measure the DNA content of propidium iodide stained fixed cells in microplates. We demonstrate that paclitaxel and vinblastine arrested CHO cells in the expected phase of the cell cycle.

Poster
Detecting the FRET Response of the GeneBLAzer® Cell Line D1 CRE-bla CHO-K1 to Agonists and Antagonists using Microplate Cytometry
The GeneBLAzer® CHO.K1-D1 cell line (Invitrogen) stably expresses both the ß-lactamase gene downstream of the cAMP response element (CRE) and the dopamine D1 receptor. Stimulation of the cells with dopamine D1 receptor agonists, results in transcriptional activation of the ß-lactamase gene through CRE. A FRET-enabled substrate (CCF4-AM) fluoresces green, in the absence of ß- lactamase reporter activity, and blue when cleaved.

Poster
Design and Cellular Engineering
TRANSAT is a research partner for conception of cellular models. Its expertise lies in manipulation of gene expression (RNAi) and in mammalian cell culture. This expertise enables the company to propose functional genomics tools and customized research services, adapted to its clients' problematic. TRANSAT maintains its innovation through R&D programs.

Poster
LacZ Reporter Fusion Assay for Rapid and Easy Identification of Highly Efficient siRNA and its Delivery by Lentivirus into Suspension Cell Lines
The LacZ reporter fusion assay is an excellent method to precisely quantify knockdown efficiency of siRNA-sequences and can be corroborated by Western blot analysis. Efficient production of Lentiviruses and high transduction efficiency (up to 98%) were achieved on lymphoid B- and T-cells as demonstrated by FACS analysis and GFP expression. Successful knockdown effect with specific shRNA was observed in suspension cells three days after lentiviral infection.

Poster
Determination of Interferon-Pathway–Related Gene Induction during RNAi
Experimental approaches using short interfering RNA (siRNA) molecules to specifically silence gene expression have become more widely used in recent years. As for all techniques in molecular and cellular biology, the importance of protocol optimization and the use of appropriate controls cannot be overestimated.

Poster
Development of High-Throughput PAMPA as an In Vitro Model of Passive Transcellular Permeation
The absorption of orally administered compounds is largely determined by their ability to cross the gastrointestinal tract. Cell culture models can be intensive and limited to a narrow pH range. Assays using artificial membranes, such as PAMPA (parallel artificial membrane permeation assay) can be used as an alternative approach to assess in vitro transcellular passive permeation.

Poster
Monitoring Translocation Activity of Transcription Factors and Nuclear Hormone Receptors Using EFC
Enzyme Fragment Complementation can provide data for analysis of protein translocation events in whole cells, with the added benefits of increased throughput, simplified luminescence detection, no antibody requirements and decreased assay costs.

Poster
From Medicinal Plants to Bioactive Drugs
The aim of our study was to analyze the antiproliferative activity effects on human tumor cell lines and the differentiating activity on human erythroleukemic K562 cells of different extracts derived from medicinal plants of Bangladesh and Lebanon.

Poster
High Throughput Automated High Content Screening using a BioCube™ System and Microplate Cytometry
Here, we model the integration of the Protedyne BioCube System and the Acumen Explorer for practical high-throughput, high content screening of protein kinases.

Poster
GPVI-RBL-2H3-NFAT-Re Luciferase Cell Line: a New Cell Based System for Studying Collagen Receptor Activation
This study describes the generation of a cell based assay which allows the functional characterization of the human GPVI receptor, by the use ofluminescence.
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