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Mass Spectrometry – Multimedia

AN INTEGRATED 1H NMR, GC-MS AND HPLC-(ESI/TOF) BASED METABOLOMICS APPROACH TO THE STUDY OF ACUTE EXERCISE IN HUMAN SERUM METABOLOME content piece image
Poster

AN INTEGRATED 1H NMR, GC-MS AND HPLC-(ESI/TOF) BASED METABOLOMICS APPROACH TO THE STUDY OF ACUTE EXERCISE IN HUMAN SERUM METABOLOME

There is an increasing concern about the different response and effect that acute exercise induces on diabetic people and a novel insight into this effect can be performed using a metabolomic approach. Metabolomic is becoming widely spread used as a new and powerful tool for discerning significant changes at metabolic level. In this study we aimed to perform a non-targeted analysis and to identify metabolic differences arisen after 30 minutes of acute exercise in both young men suffering from Ty
Fast PK and Semi Quantitative Analysis of Metabolites Using High Resolution MS content piece image
Poster

Fast PK and Semi Quantitative Analysis of Metabolites Using High Resolution MS

It has become essential to eliminate poor candidates as early as possible in the drug development process. This realization led to the application of high throughput principles to the in vivo Lead Optimization process, wherein the NCE is first given to rodents to determine the PK profile in a rapid and limited study. This rapid primary screen is known as Fast PK and several critical decisions are based on this initial study.
LC-MS Approaches to Profiling of Non-Radiolabelled Metabolites in Response to Recent Regulatory Changes content piece image
Poster

LC-MS Approaches to Profiling of Non-Radiolabelled Metabolites in Response to Recent Regulatory Changes

Following publication of the FDA MIST guidelines and revision of ICH M3, there is increasing interest in obtaining metabolic profiling data at an early stage in the development of a drug. This has led to a requirement to estimate the relative abundance of metabolites in samples prior to the synthesis of the radiolabelled compound and from a wider range of studies.
Basic amino acids profiling during hepatic tumorigenesis by hydrophilic interaction liquid chromatography tandem mass spectrometry (HILIC-MS/MS) content piece image
Poster

Basic amino acids profiling during hepatic tumorigenesis by hydrophilic interaction liquid chromatography tandem mass spectrometry (HILIC-MS/MS)

This study is aimed to characterize basic aminoacids profiles from liver tissues of primary hepatocarcinoma (HCC) and metastases from colorectalcarcinomas (MET), and from adjacent cirrhotic related hepatitis-C-virus (CIRR) and non-cirrhotic normal liver (NT). Arginine levels in MET are significantly higher (P<0.05) compared to NT, CIRR and HCC. Although our preliminary data should be validated, profiling of basic aminoacids could allow a better classification of liver malignancies in humans.
A method for the quantification of sterols for a rapid screening of Smith-Lemli-Opitz Syndrome by Atmospheric Pressure Thermal Desorption Chemical Ionization -Mass Spectrometry (APTDCI -MS) content piece image
Poster

A method for the quantification of sterols for a rapid screening of Smith-Lemli-Opitz Syndrome by Atmospheric Pressure Thermal Desorption Chemical Ionization -Mass Spectrometry (APTDCI -MS)

An atmospheric pressure thermal desorption chemical ionization MS/MS method to quantify cholesterol and 7- and 8-dehydrocholesterols directly in dried blood/plasma spots, is reported. Cholesterol levels from dried plasma spots of 23 unaffected subjects and 9 Smith-Lemli-Opitz patients by APTDCI-MS/MS method and by enzymatic method were correlated (y=0.9166x + 0.3811; r= 0.8831) as well as dehydrocholesterol levels obtained by APTDCI-MS/MS method and by GC-FID method (y=0.8214x + 0.7388; r= 0.828
Alteration of urinary metabolic profile in rats treated with methylmercury content piece image
Poster

Alteration of urinary metabolic profile in rats treated with methylmercury

A metabolomic approach was used to detect changes in urinary metabolic profile of rats treated orally with methylmercury (MeHg), a ubiquitous fish contaminant with well known neurotoxic properties. An aliquot of urine was collected on day 14, extracted and the extract successively methoxymated and trimethylsilylated prior to analysis by GC/TOF-MS. Results indicate that several metabolic pathways were altered by MeHg treatment, including urea cycle, glutathione metabolism and amino acid degradati
Minor change in the structure major change in the mass spectrum in the example of an N-toluene-pyrrolidine derivative content piece image
Poster

Minor change in the structure major change in the mass spectrum in the example of an N-toluene-pyrrolidine derivative

MS2 and further MSn spectra of SAR cannot provide sufficient information for metabolite structure elucidation because the masses of fragments in MSn correspond only to separate moieties of SAR. Nevertheless almost the exact structure of a dehydrogenated compound - has a poor MS2 spectrum - could be determined by further fragmentation in an LTQ-Orbitrap instrument and using the MSn mass spectra of an impurity of SAR.
App Note / Case Study

EPA Method 524.2: Determination of Volatile Organic Compounds in Drinking Water by Capillary Column GC/MS

EPA Method 524.2, “Measurement of Purgeable Organic Compounds in Water by Capillary Column Gas Chromatography/Mass Spectrometry”, is a general-purpose method for the identification and simultaneous measurement of purgeable volatile organic compounds in surface water, groundwater, and drinking water.
App Note / Case Study

Monitoring of volatile organic contamination in cleanroom environments

Airborne molecular contamination (AMC) is a concern for any high technology manufacturing process, especially in the microelectronics industry. Organic contamination may cause adverse effects on production tools and consequently increase costs for high-tech companies.
App Note / Case Study

Real-time Measurement of Plant Defense and leaf Wounding Reactions.

Common methods of analyzing compounds released when leaves are wounded do not have the capability to monitor reactions of the plants in a time resolution high enough to reveal underlying reaction sequences.
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