Mass Spectrometry – Multimedia

High resolution GC-TOFMS on the Pegasus GC-HRT allowed for quick and easy non-targeted screening of extractables from a common consumer product: the ubiquitous cell phone case. Most components could be identified with high similarity scores against commercial libraries. However, even when faced with low similarity scores against commercial libraries, such as in the case of Biphenol AF, the ability to rely on high mass accuracy allowed for confident identification of components.

-Source temperature was commonly the most significant variable for maximizing molecular ion peak area.
-Chromatographic peak shape was temperature dependant primarily for the non-volatile compounds.
-Ionization conditions were optimized to maximize molecular ion for a variety of compound classes.
-Ionization conditions do change the peak shape and peak area, which relates directly to library searchability.

• To demonstrate a fully automated workflow for both liquid injection and headspace for GC and GC/MS.
• Automatically optimize analysis conditions and derivatization parameters.
• Automate standard preparation and derivatization procedures.
• Reduce analysts time involved in the analysis.
• Compare manual versus automated preparation of standards.

• Automated Bligh and Dyer extraction for metabolomic studies.
• Dual-column UHPLC setup for the analysis of the polar and lipidic fractions.
• Alternating acidic and basic mobile phase for the separation of the polar fraction.
• Identification of unknown compounds by SWATH HR MS2 spectra acquisition.

The PAL RTC provides an affordable and reliable platform optimised for medium-throughput peptide preparation for shotgun-proteomics based mass spectrometry.
Reduce hands-on time on repetitive work!
Increase consistency!
Increase reproducibility

This poster has demonstrated GC-TOFMS instrumentation applied to characterize beer-related samples. The ability to isolate and identify individual analytes through chromatographic resolution, mathematical deconvolution, and mass spectral searching to library standards provides good insight to various stages of production.

Comprehensive study of regulation in myxobacteria with an untargeted metabolomics setup using Isotopic Ratio Outlier Analysis (IROA) and UHR-Q-TOF

Reliable relative quantitation of known and unknown metabolites from a myxobacterial mutant strain in response to induction of the transcriptional antirepressor taA

Compound identification facilitated by the use of ultra-high resolution MS and the knowledge of the number of carbons in each molecule due to IROA

Purpose: An overview of the results of GC amenable compounds is given for EU 2013/39/EU
Methods: For the volatile compounds headspace GCMS was used; for the semivolatiles liquid liquid
extraction was applied followed by GCMSMS detection.
Results: The detection limits are calculated by standard deviation and there are some linearity examples. Not all compounds are listed in this poster, but a full list is available.

We introduce a new, innovative injector which allows conservation of helium carrier gas. While preserving the analytical GC column flow with helium, it maintains the septum purge and the split flows with another inert gas like nitrogen - even during the analytical run. The analytical performances of the injector are in line with those of the common split/splitless injector module

Purpose: Develop a rapid, sensitive method for determining amitraz and its metabolites in milk.
Methods: Samples were hydrolyzed in acidic condition and extracted with hexane. After derivativation, the samples were analyzed by GC-ECD or directly by GC-MS.
Results: The results show that GC-MS determination of amitraz and its metabolites is rapid and sensitive.