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Screening Strategies in Drug Discovery – Multimedia

TDS LIMS: A Platform for Comprehensive Management and Analysis of Screening Data content piece image
Poster

TDS LIMS: A Platform for Comprehensive Management and Analysis of Screening Data

Here we present a web-based bioinformatics solution for the management and analysis of all areas of cell-based screen experimentation: the Laboratory Information Management System of the Technology Development Studio (TDS-LIMS). TDS-LIMS is a software package consisting of the Library Checker, Library Browser, Scheduling Kit, Screen Browser and Screen Publisher.
Determination of Cell Colony Formation in a High Content Screening Assay content piece image
Poster

Determination of Cell Colony Formation in a High Content Screening Assay

Here, laser scanning microplate cytometry has been used to provide an automated high content readout of the effects of cytostatic agents on colony formation. This approach determines colony number through the application of a spherical volume algorithm. This permits the differentiation of cytostatic effects where the number of colonies and size remains constant and cytotoxic effects where the size and number may be reduced.
High Throughput Screening of Biocompatible Polymers content piece image
Poster

High Throughput Screening of Biocompatible Polymers

The aim of this project was to develop high throughput screening methods using an IMSTAR Pathfinder™ High-content imaging platform to support the analyses of polymeric materials synthesised using a parallel approach. This was achieved by developing arrays of polymeric materials.
A Solution for Serial Dilution of Compounds in 1536-Well Microplates content piece image
Poster

A Solution for Serial Dilution of Compounds in 1536-Well Microplates

High-density screening platforms are an integral part of any successful high-throughput screening facility. The reduced assay volumes associated with increasing well density lowers reagent costs, accelerates assay throughput, and can improve assay quality and reproducibility by minimizing assay duration.
A High Content Primary Screening Assay Identifying Multiple Protein Targets in a Cell Using Quantum Dots content piece image
Poster

A High Content Primary Screening Assay Identifying Multiple Protein Targets in a Cell Using Quantum Dots

The development of multiplexing capabilities and high content readouts reporting individual cellular measurements enables assessment of biological variability on a single cell basis. A multiplexed high content screening (HCS) assay allows additional information to be gained from a single assay.
Screening and Profiling for Kinases: Development of a Unique and Versatile Platform content piece image
Poster

Screening and Profiling for Kinases: Development of a Unique and Versatile Platform

To build a unique kinase platform providing reliable and quantitative data, we have designed a flexible system with well-defined yet different assay conditions for each kinase. This platform minimizes interferences, including those from test compounds, and displays a homogeneous and generic assay format.
High Throughput Automated High Content Screening using a BioCube™ System and Microplate Cytometry content piece image
Poster

High Throughput Automated High Content Screening using a BioCube™ System and Microplate Cytometry

Here, we model the integration of the Protedyne BioCube System and the Acumen Explorer for practical high-throughput, high content screening of protein kinases.
Miniaturization of Protein Kinase Assays in the MultiScreen®HTS 384-Well P81 Phosphocellulose Filter Plate content piece image
Poster

Miniaturization of Protein Kinase Assays in the MultiScreen®HTS 384-Well P81 Phosphocellulose Filter Plate

In this study, using Protein Kinase A (PKA) as are presentative assay system, peptide phosphorylation reactions carried out in 384 PH filter plates showed dependence on enzyme and substrate concentrations, and on time. Kinase inhibition studies demonstrated IC50 and Z’ values equivalent to those obtained in 96-well plates. The higher throughput 384 well format offers a miniaturized version of the 96 well assay with equivalent sensitivity and reproducibility.
High Density Receptor Ligand Binding Assays in the MultiScreen®HTS 384-well Glass Fiber Filter Plate content piece image
Poster

High Density Receptor Ligand Binding Assays in the MultiScreen®HTS 384-well Glass Fiber Filter Plate

Using the Muscarinic M1 G-protein Coupled Receptor as a model system, we achieved accurate and reproducible determinations of binding affinity (Kd) and IC50 results for known ligands on the 384-well plate device. As a result of being able to incubate the reaction mixture in the filter plate and configure the assay using half the reaction volume, significant reductions in reagent costs and radioactive waste were achieved.
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