Quick Tips: How To Choose a Primary Antibody for Multiplex Western Blotting
In this video, we discuss the selection of primary antibodies.
- Choosing primary antibodies from different host species is often the easiest way to multiplex.
- When choosing antibodies from different host species, distantly related species are less likely to have issues with species cross-reactivities. Avoid choosing antibodies from closely related species like mouse and rat
- Using primary antibodies from the species but different antibody classes like IgG and IgM in combination with appropriate secondary antibodies is one way to overcome species issues
- Primary antibodies from the same species but of different isotypes such as IgG-1 and IgG-2 provide another possible solution
Learn more at https://www.bio-rad.com/QuickTips