Quick Tips: How To Choose a Primary Antibody for Multiplex Western Blotting

In this video, we discuss the selection of primary antibodies.

- Choosing primary antibodies from different host species is often the easiest way to multiplex.
 
- When choosing antibodies from different host species, distantly related species are less likely to have issues with species cross-reactivities. Avoid choosing antibodies from closely related species like mouse and rat

- Using primary antibodies from the species but different antibody classes like IgG and IgM in combination with appropriate secondary antibodies is one way to overcome species issues

- Primary antibodies from the same species but of different isotypes such as IgG-1 and IgG-2 provide another possible solution

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