Application Notes

 
Automated Illumina TruSeq® Nano DNA Sample Preparation
Application Note

Tecan Group Ltd.

The TruSeq Nano DNA Sample Preparation Kit enables efficient investigation of samples where the amount of DNA available is limited. This low-input method generates excellent quality data from as little at 100 ng of DNA, allowing the study of samples with restricted DNA availability, for example from tumors, and helping to preserve precious sample material for use in future studies.

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Simple sample preparation for the determination of vitamin D in serum by LC-MSMS
Application Note

Tecan Group Ltd.

A growing number of laboratories have moved from the use of immunoassay methods to liquid chromatography coupled with tandem mass spectrometry (LC-MSMS) for the determination of vitamin D. The advantage of the latter method is unparalleled specificity combined with high sensitivity, offering the potential for the determination of multiple analytes, such as 25OH-vitamin D3 and 25OH-vitamin D2.

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Automated Sample Preparation of 25-OH-Vitamin D3/D2 from Serum with the AC Extraction Plate and the Tecan Freedom EVO® for the quantification by LC-MS/MS
Application Note

Tecan Group Ltd.

There is an increasing demand for the determination of Vitamin D status which is based on the quantification of the two metabolites 25-OH-Vitamin D3/D2. Numerous laboratories have moved from the use of immunoassay methods to liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) because of its specificity combined with high sensitivity. Moreover LC-MS/MS offers the potential for multianalyte determination which is required for Vitamin D status determination.

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Automated sample preparation for the determination of testosterone in serum by LC-MSMS
Application Note

Tecan Group Ltd.

For accurate, reproducible quantification of testosterone, a growing number of laboratories are shifting from immunoassay to liquid chromatography coupled with tandem mass spectrometry. This application note describes the preparation of serum samples for subsequent LC-MS analysis of testosterone, focusing on a streamlined workflow suitable for automation.

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Colony Picking - fully automated screening of fungi
Application Note

Tecan Group Ltd.

Manual screening processes are very costly and time consuming. This ambitious project for high throughput screening (HTS) of cellulase-producing fungal strains demonstrates the high capacity and unique features of the Freedom EVO colony picking workstation. The picking of fungi is more complex than for typical bacterial colonies, and requires advanced selection criteria to be combined with complex pipetting and a tip movement that mimics the manual picking process.

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Automating ELISAs on Tecan’s Freedom EVO® using Optimiser™ technology from Siloam Biosciences
Application Note

Tecan Group Ltd.

One of the challenges in life sciences research today is to discover methods for running key assays more quickly, more reliably and using lower volumes of reagents and sample, but still with improved sensitivity. One area where this particularly holds true is for traditional enzyme-linked immunosorbent assays (ELISAs), whose application provides a useful measurement of antigens, including cytokines and a host of other biomarkers.

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Automated Illumina NGS Sample Preparation: TruSeq® Stranded mRNA on the Hamilton Microlab STAR
Application Note

Illumina’s sample preparation kits produce high quality libraries for DNA and RNA sequencing. Hamilton offers the robust Microlab STAR line of robotic liquid handling workstations for automating sample preparation. Together, the automation of the TruSeq Stranded mRNA sample preparation protocol on the STAR gives the user hours of reliable, unattended library preparation.

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Advanced Automated Library Searching for Compound Identification in Forensic Toxicology Samples
Application Note

SCIEX

LC-MS/MS utilization in forensic toxicology screening for drugs and drug metabolites has become increasingly popular due to the selectivity, sensitivity and the speed of LC-MS/MS analysis. MS/MS confirmation with automated searching against available spectral libraries has proven to add a superior level of confidence to the compound identification.

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Automated Solid Phase Extraction of THC and Metabolites from Whole Blood using the Gilson GX-274 ASPEC™
Application Note

Gilson

This application note discusses a simple and effective automated solid phase extraction method using the GX-274 ASPEC™ prior to sample analysis via LC/MS-MS.

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Automation of Solid Phase Extraction (SPE) Multi-method for GC/MS Analysis of Drugs of Abuse in Human Serum
Application Note

Gilson

This application demonstrates the extraordinary flexibility of the GX-271 ASPEC and TRILUTION LH software since the desired SPE products can be eluted in fractionated mode.

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