Automated GS FLX Titanium emPCR Enrichment using the REM e System on a Hamilton Microlab® STARlet Liquid Handler

The Genome Sequencer FLX workflow consists of three primary processes: library preparation, emPCR amplification, and sequencing (Figure 1). The REM e System is a liquid handling accessory designed to fully automate the enrichment and sequence primer annealing steps of the GS FLX Titanium emPCR process. When integrated with a liquid handling platform, the REM e System dramatically simplifies the emPCR workflow by replacing 5 hours of dedicated manual lab work with an automated walkaway procedure.

A number of common liquid handlers have been tested for compatibility with the REM e System, including the Hamilton Microlab STARlet liquid handler. Results from various samples and formats using the STARlet liquid handler, performed at the 454 Life Sciences Sequencing Center, demonstrate that together the systems achieve sequencing results comparable to those of manual emPCR enrichment. One such experiment is presented in this application note.