EMBL Course: RNA Sequencing Library Preparation - How Low Can You Go?
Event Mar 19 - Mar 23, 2018
Location: Heidelberg, Germany
Aim of this course is to prepare RNA libraries for Illumina sequencing by using the novel NEBNext Ultra II Directional RNA kit for low input applications. Throughout the course, we will be generating libraries from ultra- low as well as from normal amounts of RNA (1 ng to 50 ng) input to better understand the impact of input amounts and PCR cycles on the bioinformatics dealing with complexity, coverage, duplication rates and uniformity of libraries