MAM is a powerful LC-MS method for simultaneously monitoring and quantifying multiple product or critical quality attributes of a biotherapeutic. Traditionally, CID-based MS/MS is employed as the first step for identification, followed by quantification with either only MS or MS and MS/MS workflows. However, full sequence confirmation, including isomeric differentiation for deamidated peptides and the localization of fragile modifications, such as glycosylation, are not possible with CID-based MS/MS methodologies. Hence, the CID-based identification workflow must rely on complementary MS/MS techniques that use more advanced instrumentation in separate experiments before quantification of selected species can be performed.
Watch this webinar to discover an EAD platform method that:- Enables confident and accurate identification
- Accurately quantify and monitor quality attributes
- Can do all the above with MAM in a single injection
