Agilent Technologies’ Exome Target-Enrichment System to Analyze 1,500 Samples in Erasmus Genetic Population Study
News Mar 08, 2012
The researchers are studying reduced heterozygosity exhibited by residents of one Dutch village compared to the general population, and any relationship this has with risk factors for type-2 diabetes or cardiovascular disease. In this portion of the project, exomes from 1,500 samples will be sequenced.
“The genetically isolated population of the study is ideal for the discovery of new genetic variants related to complex disorders,” said Wilfred van IJcken, head of the genomics core facility at Erasmus Medical Center. “We are halfway through this sequencing project and are very pleased with the results so far.
The newly designed v4 exome-capture platform needs only 4 Gbp, which is financially attractive because more exomes can be analyzed. Furthermore, we have been pleasantly surprised by the quality of the data.”
“We are excited by the potential implementation of exome sequencing for clinical diagnostics as well,” van IJcken added. “We hope that this approach will be one of the favorites to succeed.”
“As interest continues to grow in exome sequencing,” said Robert Schueren, Agilent vice president and general manager, Genomics, “we’ve put a lot of effort into providing a complete, streamlined exome target-enrichment workflow from sample prep to sequencing.”
SureSelect XT includes SureSelect biotinylated RNA library “baits,” hybridization buffers, and optimized gDNA prep and library prep kits.
The investigators have produced 50 exomes per week during each of three consecutive months, including analysis. The workflow uses the Agilent Bravo liquid-handling platform to automate DNA library prep and in-solution enrichment.
The SureSelect portfolio provides a complete and customizable target-enrichment platform. Since it was introduced in 2009, SureSelect has been instrumental in more than 230 published studies covering research into a wide range of heritable disorders and complex diseases. There are now more than 23 products in the SureSelect portfolio, including a full range of catalog and custom products. The Agilent Bravo automated liquid-handling platform is the fastest and most versatile small footprint liquid-handling system available.
Novel Microplate 3D Bioprinting Platform for Engineering Muscle and Tendon TissuesNews
There is a strong need for medication that treats age-related degenerative muscle and tendon diseases. A critical bottleneck in the discovery and development of novel drugs for skeletal muscle is the lack of efficient and robust functional in vitro assays for compound screening. Researchers describe the development of a novel screening platform with automated production of 3D muscle- and tendon–like tissues using 3D bioprinting.READ MORE
Sartorius Stedim Biotech and Siemens Sign Automation AgreementNews
Siemens becomes a preferred supplier for automation solutions. Product portfolio of Sartorius Stedim Biotech to feature a globally standardized automation platform in the future.READ MORE
Automated Robotic Device Enables Faster Blood TestingNews
Rutgers researchers have created an automated blood drawing and testing device that provides rapid results, potentially improving the workflow in hospitals and other health-related institutions to allow health care practitioners to spend more time treating patients.READ MORE
Comments | 0 ADD COMMENT
International Conference on Central Nervous System and Therapeutics
Nov 12 - Nov 14, 2018