Blood-vessel-on-a-chip Provides Insight into Novel Anti-Inflammatory Drug Candidate
The blood-vessel-on-a-chip consists of parallel channels (red) lined with human endothelial cells, through which whole blood and other compounds can be perfused, to mimic the function of blood vessels in the human body. Credit: Wyss Institute at Harvard University
One of the most important and fraught processes in the human body is inflammation. Inflammatory responses to injury or disease are crucial for recruiting the immune system to help the body heal, but inflammation can also cause dangerous blood clots and other conditions by inducing an overproduction of the coagulant protein thrombin. Activated protein C (APC), a naturally occurring anti-coagulant protein with anti-inflammatory and other protective effects, is used medically to treat severe blood infections and wounds by reducing inflammation of the endothelial cells that line blood vessels. However, its use is limited because it can inhibit thrombin too much, which impacts the blood's ability to clot normally and increases bleeding risk.
Now, a collaborative team of researchers from the Division of Hemostasis and Thrombosis at Beth Israel Deaconess Medical Center (BIDMC) and the Wyss Institute at Harvard University have discovered that synthetic APC-mimicking small molecules called "parmodulins" provide anti-inflammatory and anti-thrombotic protection to endothelial cells on par with APC's without interfering with normal blood clotting and coagulation, making them attractive new drug candidates. This work was enabled by leveraging the Wyss Institute's Organ-on-a-Chip technology to model thrombosis within a human blood vessel in vitro. The results are reported in this week's issue of Proceedings of the National Academy of Sciences.
"We essentially performed a mini pre-clinical trial of parmodulins' effect on the endothelium, and not only determined the pathway through which parmodulins function, but also demonstrated that they help protect endothelial cells from inflammatory damage," says former Wyss postdoc Abhishek Jain, Ph.D., who is now an Assistant Professor and director of the Bioinspired Translational Microsystems lab at Texas A&M University.
The target protein on which both APC and parmodulins act is the transmembrane protein protease-activated receptor 1 (PAR1), which is present on both endothelial cells and platelets that circulate through the blood and promote clotting, making mechanistic analysis difficult. PAR1 was originally identified as a receptor for thrombin, which is a crucial part of the inflammatory process. However, when PAR1 is activated by APC on endothelium, it triggers anti-inflammatory, anti-apoptotic, and barrier-fortifying pathways, all of which help protect cells from the negative effects of inflammation.
In addition to activating PAR1, APC also independently inhibits the generation of thrombin, which is an essential component of healthy blood clotting - but inhibiting thrombin too much leads to uncontrolled bleeding. Knowing that parmodulins bind to PAR1, the team of scientists and clinicians set out to find a way to activate endothelial PAR1 and reduce thrombic responses without thinning the blood, and thus provide a better alternative to APC.
To evaluate the activity of parmodulins on endothelium, Karen De Ceunynck, Ph.D., postdoctoral research fellow at BIDMC and first author of the paper, incubated human endothelial cells with parmodulin 2 in vitro for 4 hours and then exposed them to the thrombin-inducing inflammatory agents lipopolysaccharide (LPS) or tumor necrosis factor-α (TNF-α). In the parmodulin-exposed cells, both agents' ability to generate thrombin was reduced by over 50% compared with non-parmodulin-exposed cells. However, parmodulin 2 did not inhibit the activity of factor V or factor X, proteins that function in blood coagulation. "We were intrigued by the notion that parmodulin 2 inhibited LPS- and TNF-mediated prothrombotic effects on the endothelial surface without impairing blood clotting" says De Ceunynck.
To confirm this theory, the team used a Wyss-developed blood-vessel-on-a-chip consisting of microfluidic channels embedded in a clear polymer chip, coated with collagen, and lined by human endothelial cells. Whole blood was perfused through the chip to simulate the flow conditions within human blood vessels, to which were added different pro- and anti-inflammatory compounds to evaluate the response of the endothelium.
When the endothelial cells were exposed to TNF-α before being perfused with whole blood, platelets accumulated on the endothelium in a typical inflammatory response; if the cells were first exposed to parmodulin 2 and then TNF-α, platelet accumulation was inhibited and the endothelium resumed its normal function. These results indicated that parmodulin exposure blocks the thrombotic response of endothelium to inflammatory stimuli without affecting blood coagulation in humans - a significant improvement over APC.
A series of tests in vitro performed by co-first author Christian Peters, Ph.D. at BIDMC, confirmed that parmodulin 2's activation of PAR1 also induces cytoprotective responses in endothelial cells by inhibiting apoptosis (programmed cell death) induced by thrombin, TNF-α, and the apoptotic alkaloid staurosporine through a signaling pathway that begins with parmodulin 2's binding to a specific site on the cytoplasmic side of PAR1. "We observed that the cytoprotective response induced by parmodulin 2 happened very quickly, and confirmed its rapid onset in time course and gene expression assays," says Peters.
Additionally, in vivo studies in mice showed that parmodulin 2 reduces the binding of white blood cells to blood vessels and impairs platelet and fibrin accumulation at injury sites during the inflammatory response, confirming the anti-thrombotic and anti-coagulant activity of parmodulin 2 observed in vitro. Additionally, parmodulins do not interact with many of APC's other binding partners, making it much more targeted to PAR1 and reducing other side effects.
"The discovery of an anti-inflammatory molecule that prevents endothelial thrombosis but also preserves normal blood coagulation is a major step toward an alternative and better approach to treating inflammatory disease," says Rob Flaumenhaft, M.D., Ph.D., Professor of Medicine at Harvard Medical School, Chief of the Division of Hemostasis and Thrombosis at BIDMC, and corresponding author of the paper. "Furthermore, nearly all other pharmaceuticals that target transmembrane PAR1-like receptors bind to the exterior side of the receptor; parmodulin 2 represents a paradigm shift for compounds targeting these receptors because it acts on the cellular side of the protein. We are excited to see if we can advance it to clinical trials."
"This work provides another example of how organ-on-a-chip technology can enable faster and safer development and evaluation of drugs that could help patients around the world," says co-author and Wyss Institute Founding Director Donald Ingber, M.D., Ph.D., who is also the Judah Folkman Professor of Vascular Biology at HMS and the Vascular Biology Program at Boston Children's Hospital, as well as Professor of Bioengineering at Harvard's John A. Paulson School of Engineering and Applied Sciences (SEAS).
Additional authors of the paper include Sarah Higgins, Ph.D., also a Research Fellow at BIDMC; Omozuanvbo Aisiku, Ph.D, former Postdoctoral Research Fellow in the Division of Hemostasis and Thrombosis at BIDMC and currently a scientist at Instrumentation Laboratory; Jennifer Fitch-Tewfik, Ph.D., former Postdoctoral Research Fellow in the Division of Hemostasis and Thrombosis at BIDMC and currently a teacher at Southeastern Regional Vocational Technical High School; Sharjeel Chaudhry, a Predoctoral Fellow in the Division of Hemostasis and Thrombosis at BIDMC; Chris Dockendorff, Ph.D., Assistant Professor at Marquette University; and Samir Parikh, M.D., Associate Professor at HMS.
This research was supported by the National Heart, Lung, and Blood Institute and the Wyss Institute for Biologically Inspired Engineering at Harvard University.
This article has been republished from materials provided by Wyss Institute. Note: material may have been edited for length and content. For further information, please contact the cited source.
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