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Building Protein-Protein Interaction Networks with Proteomic and Informatics Tools
Abstract
The systematic characterization of the whole interactomes of different model organisms has revealed that the eukaryotic proteome is highly interconnected. Therefore, biological research is progressively shifting away from classical approaches which focus only on a few proteins towards whole protein interaction networks to describe the relationship of proteins in biological processes. In this mini-review, we survey the most common methods for the systematic identification of protein interactions, and exemplify different strategies for the generation of protein interaction networks.
The article is published online in the Journal of Biological Chemistry and is free to access.
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