Catching a Big Fish Genome Requires Big Data
Catching a Big Fish Genome Requires Big Data
If you eat fish in the U.S., chances are it once swam in another country. That's because the U.S. imports over 80 percent of its seafood, according to estimates by the United Nations. New genetic research could help make farmed fish more palatable and bring America's wild fish species to dinner tables. Scientists have used big data and supercomputers to catch a fish genome, a first step in its sustainable aquaculture harvest.
Researchers assembled and annotated for the first time the genome - the total genetic material - of the fish species Seriola dorsalis. Also known as California Yellowtail, it's a fish of high value to the sashimi, or raw seafood industry. They published their results on January of 2018 in the journal BMC Genomics.
"The major findings in this publication were to characterize the Seriola dorsalis genome and its annotation, along with getting a better understanding of sex determination of this fish species," said study co-author Andrew Severin, a Scientist and Facility Manager at the Genome Informatics Facility of Iowa State University.
"We can now confidently say," added Severin, "that Seriola dorsalis has a Z-W sex determination system, and that we know the chromosome that it's contained on and the region that actually determines the sex of this fish." Z-W refers to the sex chromosomes and depends on whether the male or female is heterozygous (XX,XY or ZZ,ZW), respectively. Another way to think about this is that in Z-W sex determination, the DNA molecules of the fish ovum determine the sex of the offspring. By contrast, in the X-Y sex determination system, such is found in humans, the sperm determines sex in the offspring.
It's hard to tell the difference between a male and female yellowtail fish because they don't have any obvious phenotypical, or outwardly physically distinguishing traits. "Being able to determine sex in fish is really important because we can develop a marker that can be used to determine sex in young fish that you can't determine phenotypically," Severin explained. "This can be used to improve aquaculture practices." Sex identification lets fish farmers stock tanks with the right ratio of males to females and get better yield.
Assembling and annotating a genome is like building an enormous three-dimensional jigsaw puzzle. The Seriola dorsalis genome has 685 million pieces - its base pairs of DNA - to put together. "Gene annotations are locations on the genome that encode transcripts that are translated into proteins," explained Severin. "Proteins are the molecular machinery that operate all the biochemistry in the body from the digestion of your food, to the activation of your immune system to the growth of your fingernails. Even that is an oversimplification of all the regulation."
Severin and his team assembled the genome of 685 megabase (MB) pairs from thousands of smaller fragments that each gave information to form the complete picture. "We had to sequence them for quite a bit of depth in order to construct the full 685 MB genome," said study co-author Arun Seetharam. "This amounted to a lot of data," added Seetharam, who is an associate scientist at the Genome Informatics Facility of Iowa State University.
The raw DNA sequence data ran 500 gigabytes for the Seriola dorsalis genome, coming from tissue samples of a juvenile fish collected at the Hubbs SeaWorld Research Institute in San Diego. "In order to put them together," Seetharam said, "we needed a computer with a lot more RAM to put it all into the computer's memory and then put it together to construct the 685 MB genome. We needed really powerful machines."
That's when Seetharam realized that the computational resources at Iowa State University at the time weren't sufficient get the job done in a timely manner, and he turned to XSEDE, the eXtreme Science and Engineering Discovery Environment funded by the National Science Foundation. XSEDE is a single virtual system that scientists can use to interactively share computing resources, data and expertise.
"When we first started using XSEDE resources," explained Seetharam, "there was an option for us to select for ECSS, the Extended Collaborative Support Services. We thought it would be a great help if there were someone from the XSEDE side to help us. We opted for ECSS. Our interactions with Phillip Blood of the Pittsburgh Supercomputing Center were extremely important to get us up and running with the assembly quickly on XSEDE resources," Seetharam said.
"This experiment started with a collaboration with the Southwest Fisheries Science Center of NOAA," Severin explained. He said the project originally set out to complete a large RNA-seq project, and it turned out that there was sufficient funding to also do a genome assembly.
"That resulted in a long-term collaboration with the Southwest Fisheries Science Center," Severin said. "With the recent advances in high-throughput DNA sequencing, we're now able to generate terabytes of sequencing data. This tends to be short, 100-150 base pair reads that we have to put together like a very large puzzle and figure out where all the pieces go," he added.
Severin and Seetharam's team have completed the basic picture of the genome for Seriola dorsalis, but they say there's still room for refinement. "The genome that we assembled is not perfect, in the sense that it is still in many pieces. We weren't able to fully piece together entire chromosomes," explained Seetharam. "We have many scaffolds representing each of those chromosomes, and we are missing a lot of information that is needed to fill in the gaps." Sequencing technology advancements can address these gaps, Seetharam said, through the advancement of sequencing technology that can produce longer DNA reads.
Both Severin and Seetharam are resolute in their conviction that big data can solve problems in sustainable food production. "I believe the public is going to see more of this type of big data utilization and to see why science is so important for our future," Severin said. Gene annotation, he feels, is just the tip of the iceberg. "We're going to start comparing genome assemblies with each other and start getting at what a genome is and how it works; and how for a particular genome does the presence or absence of genes or its context with regard to its three-dimensional structure, how does that make a species," Severin said.
"Big data keeps getting bigger, and we're finding answers to really interesting questions." Severin concluded. Seetharam added that "There will be more studies using big data that will have significant impactful for the general public. This level of research will foster even larger studies in the future."
This article has been republished from materials provided by the Texas Advanced Computing. Note: material may have been edited for length and content. For further information, please contact the cited source.
Reference: Purcell, C. M., Seetharam, A. S., Snodgrass, O., Ortega-García, S., Hyde, J. R., & Severin, A. J. (2018). Insights into teleost sex determination from the Seriola dorsalis genome assembly. BMC Genomics, 19, 31. https://doi.org/10.1186/s12864-017-4403-1