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CRISPRi Screens Reveal Sources of Metabolic Robustness in E. coli

News   Nov 25, 2020 | Original story from the Max Planck Institute for terrestrial Microbiology

 
CRISPRi Screens Reveal Sources of Metabolic Robustness in <i>E. coli</i>

CRISPR interference uses a deactivated Cas9 protein (dCas9) and a single guide RNA (sgRNA) and enables specific knockdowns of enzymes in the metabolic network of bacteria. Deep sequencing can track growth of 7177 CRISPRi in parallel and informs about robustness of each CRISPRi strain. Credit: Max Planck Institute for terrestrial Microbiology/Link.

 
 
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