Proteomics for Systems Toxicology
News Nov 24, 2014
Current toxicology studies frequently lack measurements at molecular resolution to enable a more mechanism-based and predictive toxicological assessment. Recently, a systems toxicology assessment framework has been proposed, which combines conventional toxicological assessment strategies with system-wide measurement methods and computational analysis approaches from the field of systems biology. Proteomic measurements are an integral component of this integrative strategy because protein alterations closely mirror biological effects, such as biological stress responses or global tissue alterations. Here, we provide an overview of the technical foundations and highlight select applications of proteomics for systems toxicology studies. With a focus on mass spectrometry-based proteomics, we summarize the experimental methods for quantitative proteomics and describe the computational approaches used to derive biological/mechanistic insights from these datasets. To illustrate how proteomics has been successfully employed to address mechanistic questions in toxicology, we summarized several case studies. Overall, we provide the technical and conceptual foundation for the integration of proteomic measurements in a more comprehensive systems toxicology assessment framework. We conclude that, owing to the critical importance of protein-level measurements and recent technological advances, proteomics will be an integral part of integrative systems toxicology approaches in the future.
The review is published online in Computational and Structural Biotechnology Journal and is free to access.
Reducing Painkiller Side Effects With Brain InsightsNews
Opioids are powerful painkillers that act on the brain, but they have a range of harmful side effects including addiction. Using mass spectrometry, researchers have developed a tool that gives deeper insights into the brain’s response to opioids.READ MORE
Biorenewable, Biodegradable Plastic Alternative ProgressNews
Polymer chemists have taken another step toward a future of high-performance, biorenewable, biodegradable plastics.READ MORE
Streaming Protocol Makes Gene Data Sharing Future-ProofNews
The Large Scale Genomics Work Stream of the Global Alliance for Genomics and Health (GA4GH) has announced eight new implementations of its htsget protocol, a standard released in October 2017 for accessing large-scale genomic sequencing data online that does not depend on file transfers. The protocol and interoperability testing are reported in a paper released online this week in the journal Bioinformatics.